Figure 1.

N⁶-threonylcarbamoyladenosine (t⁶A) is harbored in position 37 of A-starting codons (ANN)-pairing transfer RNAs (tRNAs). The schematic representation of the anticodon loop of an ANN-pairing tRNA: t⁶A at position 37 is represented as a red full circle; the anticodon is represented in yellow; and the rest of the tRNA in light blue. Messenger RNA (mRNA) is represented at the bottom in light blue circles and the codon in yellow. Codon-anticodon interactions are depicted with dotted lines, and are stabilized by t⁶A, thus preventing intra-loop bonding between positions U33 and A37.