Table 3.
Bacterial strains, plasmids, and primers used for construction of reporter strains.
| Strain, plasmid, or oligonucleotide | Relevant characteristics1 or sequences (5′ 3′) | Reference |
|---|---|---|
| Pseudomonas protegens | ||
| CHA0 | Wild type; biocontrol agent; DAPG+, PRN+, HCN+ | Stutz et al., 1986 |
| CHA0-gfp | CHA0::attTn7-gfp; Gmr | Péchy-Tarr et al., 2013 |
| Pseudomonas chlororaphis | ||
| PCL1391 | Wild type; biocontrol agent; HCN+, PHZ+ | Chin-A-Woeng et al., 1998 |
| PCL1391-gfp | PCL1391::attTn7-gfp; Gmr | This study |
| Escherichia coli | ||
| DH5α | Laboratory strain | Sambrook and Russell, 2001 |
| Plasmids | ||
| pBK-miniTn7-gfp2 | pUC19-based delivery plasmid for miniTn7-gfp2; mob+ Gmr Cmr Aprr | Koch et al., 2001 |
| pME7116 | prnA-gfp transcriptional fusion; reporter of PRN biosynthetic gene expression in CHA0;Tcr | Baehler et al., 2005 |
| pME9010 | mCherry-based promoter-probe vector derived from pPROBE’-gfp (AAV); Kmr | Rochat et al., 2010 |
| pME9011 | hcnA-mcherry transcriptional fusion; reporter of HCN biosynthetic gene expression in CHA0; Kmr | Rochat et al. (2010) |
| pME9012 | phlA-mcherry transcriptional fusion; reporter of DAPG biosynthetic gene expression in CHA0; Kmr | Rochat et al., 2010 |
| pME11011 | prnA-mcherry transcriptional fusion; reporter of PRN biosynthetic gene expression in CHA0; Kmr | This study |
| pME11017 | phzA-mcherry transcriptional fusion; reporter of PHZ biosynthetic gene expression in PCL1391; Kmr | This study |
| pUK21 | Cloning vector; Kmr | Vieira and Messing, 1991 |
| pUX-BF13 | Helper plasmid encoding Tn7 transposition functions; R6K-replicon; Apr | Bao et al., 1991 |
| Oligonucleotides2 | ||
| P5 BamHI new | CGGGATCCCGGGCTCAAGGACAGTTGGTTCA, BamHI | This study |
| P6 | GGAATTCCCGAGGTACGAAGCGGCCATC, EcoRI | Baehler et al., 2005 |
| phzAF | CGGGATCCCTAACTCCATTTTGAGCACC, BamHI | This study |
| phzAR | CGAGCTCGCTCAATCTCCAATGAATAAGGGGGCT, SacI | This study |
1Abbreviations: Apr, ampicillin; Cmr, chloramphenicol; Gmr, gentamicin; Kmr, kanamycin; and Tcr, tetracycline resistance, respectively.
2Restriction sites are underlined.