. 2017 Apr 1;33(4):395–403. doi: 10.1089/aid.2016.0073

Table 2.

Ex Vivo Challenge Assay Methods for Rectal (R1–R8), Cervical (C1–C4), and Vaginal (V1–V5) Data Sets: Supernatant Volume, HIV-1 BaL Source, Tissue and Forceps Details

Data set	Supernatant Vol (μl)	HIV-1 BaL source	Mean wt/size (pre/post)	Forceps brand; Manufacturer	Forceps size, mm
R1^a	400	NIH, Catalog No. 510	20–30 mg (pre)	Radial Jaw 4; Boston Scientific	3.8
R2^a	400	NIH, Catalog No. 510	20–30 mg (pre)	Radial Jaw 4; Boston Scientific	3.8
R3^a	500	Advanced	15–20 mg (pre)	Radial Jaw 4; Boston Scientific	2.8
R4^a	500	Advanced	15–20 mg (pre)	Radial Jaw 4; Boston Scientific	2.8
R5^a	500	Advanced	15–20 mg (pre)	Radial Jaw 4; Boston Scientific	2.8
R6^a	200	NIH	3 × 3 × 1 mm (pre)	Sarratt; Stericom	4
R7^a	400	NIH, Catalog No. 510	20–30 mg (pre)	Radial Jaw 4; Boston Scientific	3.8
R8^b	500	NIH, Catalog No. 510	20–30 mg (pre)	Radial Jaw 4; Boston Scientific	3.8
C1⁴²	700	Advanced	9–30 mg (post)	Tischler; BD	2.3 × 4.2
C2⁴²	700	Advanced	9–30 mg (post)	Tischler; BD	2.3 × 4.2
C3^a	500	Advanced	15–20 mg (pre)	Radial Jaw 4; Boston Scientific	2.8
C4⁴²	700	Advanced	4–13 mg (post)	Tischler; BD	2.3 × 4.2
V1⁴²	700	Advanced	11–26 mg (post)	Tischler; BD	2.3 × 4.2
V2⁴²	700	Advanced	11–26 mg (post)	Tischler; BD	2.3 × 4.2
V3^a	500	Advanced	15–20 mg (pre)	Radial Jaw 4; Boston Scientific	2.8
V4⁴³	100	NIH	30 mg (pre)	Tischler; BD	3 × 5
V5^a	100	NIH	3 × 3 × 1 mm (pre)	Sarratt; Stericom	4

HIV-1 BaL Sources: NIH = NIH AIDS Research and Reference Reagent Program, Bethesda, MD (www.aidsreagent.org/) Advanced = Advanced Biotechnologies, Inc. (https://abionline.com/).

TCID₅₀ titration methods were:

^{^a}

www.ncbi.nlm.nih.gov/pubmed/21116800

^{^b}

Titrated on activated peripheral blood mononuclear cells. Calculated by Reed-Muench Formula.