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. 2017 Apr 1;27(2):87–94. doi: 10.1089/nat.2016.0655

FIG. 4.

FIG. 4.

Mechanism of inhibition of c9orf72 intronic sense and antisense foci. Inhibition of c9orf72 intronic sense foci by ss-siRNAs. (A) List of ss-siRNAs. (B) Quantitation of microscopy showing the percentage of cells with sense RNA foci and the number of foci per 100 cells. (C) Quantitative polymerase chain reaction showing levels of sense intron 1 and mRNA after treating with 50 nM of ss-siRNAs. E34 is a duplex RNA targeting the c9orf72 coding region. ss-siRNAs were transfected into patient-derived fibroblast cells with lipid RNAiMAX (Life Technologies) as previously described [19].