FIG. 6.

EPLCs have the potential to differentiate into CF-like cells. (A) Schematic of the protocol used for CF-like cell induction. (B) qRT-PCR analysis of expression of the fibroblast markers POSTN and FSP1 in hESCs, HFFs, and EPL-derived cells. Gene expression was normalized to TBP. Error bars represent SEM; n = 3; **p < 0.01 versus untreated cultures, analyzed by the Student's t-test. (C) Flow cytometry analysis of the proportion of POSTN⁺ cells in untreated and bFGF+S-induced cultures. Error bars represent SEM; n = 3; **p < 0.01. (D) Immunofluorescence staining of POSTN and COL-1 in untreated and bFGF+S-induced cultures. Scale bars, 100 μm. CF, cardiac fibroblast; HFFs, human foreskin fibroblasts. Color images available online at www.liebertpub.com/scd