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. 2017 Mar 29;8:188. doi: 10.3389/fphys.2017.00188

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Copyright © 2017 Liu, Wang, Chen, Huang, Yang, Ahmed, Wang, Liu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photographs of TUNEL staining in April (A,B), July (C), and October (D) as well as negative control (E) of the testis and the counting of TUNEL-positive cells (F). Arrowhead, spermatogonia; Double arrowhead, spermatocyte; Double arrow, spermatid; Arrow, spermatozoa; Lc, Leydig cell; Bv, blood vessel; Lu, lumen. Scale bar = 100 μm (E), 50 μm (A,C,D) and 20 μm (B). The values (^*) obtained in April were significantly higher (P < 0.05) than those obtained in July and October.