Table 1.
Profiling of melanoma-derived extracellular vesicle (EV) protein cargo: Venn diagram summarizing the number of unique proteins identified in EVs isolated from 3 melanoma cell lines of which 21 proteins were shared among all 3 EV preparations (A); total peptide count of the 21 proteins identified in the shared signature and associated roles in dendritic cell (DC) maturation (B).
| (A) |  |
(B) | Protein name |
Peptide count |
Functional role in dendritic cell (DC) maturation | Reference | ||
| SKMEL28 | C32TG | A375 | ||||||
| S100A8 | 5 | 5 | 4 | Inhibitory | (30) | |||
| S100A9 | 3 | 5 | 3 | Inhibitory | (31) | |||
| Annexin A1 | 4 | 9 | 12 | Inhibitory | (32) | |||
| Annexin A2 | 23 | 26 | 10 | Inhibitory | (33) | |||
| Heat shock cognate protein 71 kDa | 17 | 12 | 19 | Activating | (35) | |||
| Fibronectin | 10 | 14 | 26 | Activating | (36) | |||
| Laminin subunit gamma 1 | 15 | 16 | 9 | Activating | (36) | |||
| Prolactin-inducible protein | 2 | 6 | 3 | Activating | (37) | |||
| Procollagen-lysine | 4 | 17 | 9 | Activating | (38) | |||
| ICAM1 | 6 | 4 | 4 | Inhibitory/activating | (34, 39) | |||
| Annexin A5 | 11 | 21 | 3 | – | ||||
| Chloride intracellular channel protein | 2 | 4 | 3 | – | ||||
| Lysozyme C | 3 | 4 | 3 | – | ||||
| Alpha enolase | 2 | 22 | 15 | – | ||||
| Glyceraldehyde-3-phosphate dehydrogenase | 3 | 17 | 6 | – | ||||
| Glucose 6 phosphate isomerase | 2 | 12 | 5 | – | ||||
| Glutathione S transferase | 2 | 5 | 4 | – | ||||
| Fructose bisphosphate aldolase | 9 | 16 | 7 | – | ||||
| Gelsolin | 3 | 6 | 7 | – | ||||
| Tenascin | 52 | 11 | 16 | – | ||||
| 14-3-3 Protein zeta delta | 6 | 12 | 4 | – | ||||