Figure 3.

Evaluating mutants lacking established filamentation regulatory pathways for ethanol-inducible invasion. (A) Wild-type cells (PC538, Σ1278b MATa haploid) and the indicated isogenic mutants were spotted onto nitrogen-limited medium (SLAD) with or without 2% ethanol (v/v). Plates were incubated for 4 days at 30°C, photographed, washed in stream of water, and photographed again. Bar, 5 mm. (B) Quantitation of invasive growth in panel (A) by densitometry, performed as described in the legend for Figure 1B. Cells were spotted in triplicate, and the average values are shown. Error bars represent the standard difference between experiments. Asterisk denotes a p < 0.01 for samples relative to each strain's invasion in SLAD. (C) Colony peripheries from the plates in panel (A) were examined at 20X magnification. Bar, 50 μm. Arrows mark examples of pseudohyphae.