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. 2017 Feb 27;9(3):201. doi: 10.3390/nu9030201

Figure 5.

Figure 5

(A) Representative image of the zymographic profiles of MMP-9 and MMP-2 activities in presence to different cooked and uncooked fractions isolated from soy seeds. MMP-9 and MMP-2 proteolytic activities were detected in HT29 extracellular media after a 48 h exposure to 100 µg protein/mL cooked (P C) and uncooked (P) fractions and 10 mg non-protein/mL cooked (NP C) and uncooked (NP) fractions isolated from Glycine max seeds. HT29 extracellular media were loaded in 12.5% (w/v acrylamide) polyacrylamide gels co-polymerized with 1% (w/v) gelatin; (B) Proteolytic activity of gelatinases present in the HT29 extracellular media after a 48 h exposure to 100 µg protein/mL cooked (P C) and uncooked (P) fractions and to 10 mg non-protein/mL cooked (NP C) and uncooked (NP) fractions isolated from Glycine max seeds, as quantified by the DQ fluorogenic method. Results are expressed as relative fluorescence as a percent of controls and represent an average of at least three replicate experiments (n = 3) ± SD. * represents p < 0.05 between samples and # represents p < 0.05 compared to controls.