Table 2.
Ex vivo bone mineral density (BMD), structure and peak load of the femur in 6-month-old female mice whose mothers were exposed to control (CON) or 0.5% hesperidin (HSP) + 0.25% naringin (NAR) diets through pregnancy and lactation.
| CON | HSP + NAR | p Value | |
|---|---|---|---|
| Femur BMD (g/cm2) | 0.092 ± 0.002 | 0.090 ± 0.003 | 0.427 |
| Femur Neck Trabecular Structure | |||
| BV/TV (%) | 15.6 ± 0.7 | 15.0 ± 0.9 | 0.621 |
| Tb.Th (mm) | 0.098 ± 0.002 | 0.099 ± 0.002 | 0.835 |
| Tb.N (1/mm) | 1.591 ± 0.083 | 1.523 ± 0.102 | 0.610 |
| Tb.Sp (mm) | 0.426 ± 0.014 | 0.440 ± 0.013 | 0.462 |
| Femur Midpoint Cortical Structure | |||
| Ct.Ar (mm2) | 1.312 ± 0.034 | 1.181 ± 0.076 | 0.101 |
| Ct.Th (mm) | 0.264 ± 0.009 | 0.252 ± 0.010 | 0.422 |
| Distal Femur Trabecular Structure | |||
| BV/TV (%) | 11.0 ± 0.9 | 10.8 ± 2.8 | 0.932 |
| Tb.Th (mm) | 0.081 ± 0.002 | 0.078 ± 0.005 | 0.520 |
| Tb.N (1/mm) | 1.361 ± 0.105 | 1.294 ± 0.292 | 0.813 |
| Tb.Sp (mm) | 0.463 ± 0.031 | 0.544 ± 0.069 | 0.324 |
| Femur Neck Peak Load (N) | 23.5 ± 1.2 | 24.3 ± 1.8 | 0.697 |
| Midpoint Peak Load (N) | 25.5 ± 1.1 | 25.4 ± 2.4 | 0.602 |
There were no significant differences in femur outcomes between CON and HSP + NAR groups. Data are expressed as mean ± standard error of the mean (SEM), n = 5–9/group. BV/TV = bone volume fraction, Ct.Ar = cortical bone area, Ct.Th = cortical thickness, Tb.Th = trabecular thickness, Tb.N = trabecular number, Tb.Sp = trabecular separation.