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. 2017 Mar 10;9(3):261. doi: 10.3390/nu9030261

Figure 5.

Figure 5

IL-10 production in differentiated THP-1 M2 macrophages. THP-1 M0 macrophages were exposed to SDA, ETA and EPA (50 µM) during differentiation to M2-like macrophages. PUFA-exposed M2-like macrophages were then stimulated with 1% phosphate buffered saline (Baseline) or lipopolysaccharide (10 µg/mL, +LPS) for 24 h. Cell media was collected and IL-10 concentration determined by bead-based immunoassay. Data are mean ± SEM, n = 3–5 per condition. Two-way ANOVA followed by Tukey’s multiple comparison test probed for PUFA and LPS stimulation effects. * LPS stimulation effect, p < 0.01; PUFA conditions without a common letter are significantly different, p < 0.05.