Table 4.
Analysis of rat cecal microbiota following four days of treatment diets; Study 2 1.
| Diet | Reads Obtained from MiSeq (Min~Max) | Median Seqs per Sample after Quality Filtering (Min~Max) | % OTUs Assigned | Chao1 | Observed OTUs |
|---|---|---|---|---|---|
| CON | 13,242~45,673 | 10,917~37,747 | 99.88 ± 0.07 | 3627.1 a ± 366.6 | 1573.0 a ± 77.9 |
| CB | 11,516~29,303 | 9,447~24,029 | 99.89 ± 0.07 | 3847.5 a ± 208.8 | 1576.1 a ± 23.1 |
| GRP | 14,829~29,988 | 11,900~24,483 | 99.91 ± 0.05 | 3783.7 a ± 442.5 | 1557.4 a ± 122.1 |
| CB-H | 12,761~116,686 | 10,441~96,729 | 99.92 ± 0.04 | 3968.5 b ± 172.1 | 1663.6 b ± 57.4 |
1 Microbial analysis by Illumina® 16S rRNA sequencing (V3–V5 hypervariable region). Rats (n = 32) fed four days of control (CON), cooked broccoli (CB), GRP, or GRP-free (CB-H) diet. Values within columns with the same letter are not different (p < 0.05). Rarefaction was performed based on 7410 sequences per sample when the maximum number of OTUs were observed in all groups.