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. 2017 Mar 16;9(3):288. doi: 10.3390/nu9030288

Figure 3.

Figure 3

Effect of treatment with protocatechuic acid (PCA) on inflammation-related genes in mice with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. mRNA expression of IL-6, TNF-α, IL-1β and COX-2 genes (A). Representative sections of COX-2 immunohistochemistry in colonic tissues from C, C + PCA, TNBS, TNBS + PCA30 and TNBS + PCA60, and quantification of the positive cells. Image analysis was performed using the ImageJ software v3.91 with ten non-overlapping randomly chosen histological fields. Photographs are taken under magnification ×200 (B). Data are expressed as the means ± SEM of six mice per group. a p < 0.05 vs. C group; b p < 0.05 vs. TNBS group; c p < 0.05 vs. TNBS + PCA30 group.