Table 2.
Metabolite | Fasting State | 30 min Postprandially | 420 min Postprandially | Pooled SEM | p-Value | |||||
---|---|---|---|---|---|---|---|---|---|---|
CON | TGS | CON | TGS | CON | TGS | Diet | Time | D × T | ||
Amino acids | ||||||||||
Alanine | 589.5 cd | 540.1 d | 838.6 a | 721.4 b | 645.0 bcA | 539.2 cdB | 45.23 | 0.006 | <0.001 | 0.586 |
Arginine | 160.1 bc | 166.1 b | 229.5 a | 212.0 a | 147.1 bc | 127.1 c | 12.53 | 0.306 | <0.001 | 0.482 |
Asparagine | 81.6 c | 79.1 c | 146.5 a | 138.1 ab | 123.9 b | 125.2 b | 7.32 | 0.532 | <0.001 | 0.709 |
Aspartate | 23.9 a | 15.0 b | 24.1 a | 12.7 b | 17.4 ab | 11.5 b | 3.16 | 0.001 | 0.199 | 0.627 |
Citrulline | 84 | 90.8 | 94.2 | 86.6 | 103.6 | 98.7 | 11.75 | 0.778 | 0.188 | 0.609 |
Glutamine | 962 cd | 884 d | 1139 a | 1008 bc | 1086 ab | 990 bd | 58.9 | 0.008 | 0.002 | 0.797 |
Glutamate | 138.1 b | 106.6 c | 168.0 a | 118.3 bc | 133.8 b | 97.2 c | 11.77 | <0.001 | 0.008 | 0.539 |
Glycine | 1710 | 1805 a | 1825 ad | 1822 ab | 1519 c | 1537 cd | 128.4 | 0.671 | 0.014 | 0.864 |
Histidine | 42.3 d | 69.4 c | 104.8 a | 116.6 a | 86.0 b | 115.0 a | 7.09 | <0.001 | <0.001 | 0.233 |
Isoleucine | 177.2 c | 178.8 c | 303.5 a | 282.4 a | 228.5 b | 225.4 b | 13.66 | 0.497 | <0.001 | 0.65 |
Leucine | 238.2 c | 248.1 c | 446.6 aA | 401.0 aB | 333.2 b | 322.4 b | 18.59 | 0.314 | <0.001 | 0.308 |
Lysine | 322.1 d | 366.7 d | 599.8 a | 628.4 a | 435.3 c | 518.8 b | 31.22 | 0.016 | <0.001 | 0.493 |
Methionine | 44.0 b | 47.8 b | 105.7 a | 97.7 a | 92.9 a | 96.3 a | 5.12 | 0.946 | <0.001 | 0.336 |
Ornithine | 121.9 c | 128.0 c | 182.6 a | 168.1 ab | 145.6 bc | 155.6 b | 10.63 | 0.946 | <0.001 | 0.347 |
Phenylalanine | 55.9 c | 62.3 c | 142.5 a | 131.6 a | 112.1 b | 116.4 b | 6.38 | 0.995 | <0.001 | 0.203 |
Proline | 361.9 b | 373.1 b | 655.9 a | 602.4 a | 607.1 a | 649.4 a | 29.83 | 0.999 | <0.001 | 0.11 |
Serine | 161.5 c | 158.4 c | 252.3 a | 237.8 ab | 215.7 b | 220.8 b | 10.63 | 0.568 | <0.001 | 0.503 |
Threonine | 209.6 c | 247.3 bc | 311.9 a | 305.0 a | 281.7 ab | 302.2 a | 21.99 | 0.277 | <0.001 | 0.445 |
Tryptophan | 41.8 dB | 50.8 dA | 87.5 a | 85.7 ab | 63.6 c | 77.0 b | 4.22 | 0.023 | <0.001 | 0.073 |
Tyrosine | 221.4 c | 196.3 c | 330.2 ab | 290.0 b | 349.7 a | 325.3 ab | 32.93 | 0.059 | <0.001 | 0.87 |
Valine | 487.6 c | 478.4 c | 672.6 aA | 611.1 abB | 586.1b | 566.7 b | 25.22 | 0.105 | <0.001 | 0.412 |
Biogenic amines | ||||||||||
α-AAA | 15.7 c | 32.1 a | 12.2 cd | 26.5 b | 8.2 d | 14.4 c | 2.63 | <0.001 | <0.001 | 0.006 |
Carnosine | 16.2 | 14.5 | 16.2 | 13.9 | 17 | 14.4 | 1.82 | 0.043 | 0.848 | 0.939 |
Met-SO | 3.6 d | 3.4 d | 7.1 c | 6.5 c | 9.5 b | 12.8 a | 0.52 | 0.037 | <0.001 | 0.001 |
Putrescine | 0.72 a | 0.66 ac | 0.70 a | 0.57 bcd | 0.60 abd | 0.54 d | 0.043 | 0.027 | 0.03 | 0.611 |
Sarcosine | 1.1 b | 1.2 b | 1.5 b | 0.9 b | 1.7 b | 3.1 a | 0.4 | 0.335 | 0.002 | 0.041 |
t4-OH-Pro | 95.1 bc | 91.8 bc | 112.7 a | 96.6 b | 87.0 bc | 81.1 c | 6.12 | 0.053 | 0.001 | 0.379 |
Taurine | 30.9 bc | 32.1 b | 44.8 a | 27.2 bcd | 20.3 c | 17.3 d | 4.05 | 0.052 | <0.001 | 0.045 |
SEM, standard error of the mean; D × T, Diet × Time; α-AAA, α-amino adipate; Met-SO, methionine-sulfoxide; t4-OH-Pro, trans-4-hydroxyproline. All values are presented as least square means ± SEM; CON diet, n = 6; TGS diet, n = 7. Means with different superscript letters within a row differed significantly (p < 0.05) over all sampling time points. Means with different capital superscript letters tended to differ (0.05 < p ≤ 0.10) over all sampling time points.