Table I. Statistics of small RNA sequencing data analysis.
Library Name | Total Raw Reads | Adapter Trimminga | Contaminant Filteringa | Noncoding RNA Filteringb | Unique Reads Matching the Grape Genomec |
---|---|---|---|---|---|
CS IRR L | 20,579,159 | 16,922,667 | 16,053,719 | 9,680,892 | 1,365,464 |
CS DS L | 23,614,667 | 15,125,262 | 13,881,849 | 6,290,048 | 679,650 |
CS IRR R | 16,015,312 | 13,289,525 | 11,884,302 | 6,197,784 | 247,795 |
CS DS R | 13,677,360 | 8,040,667 | 7,188,734 | 2,985,063 | 67,588 |
M4 IRR L | 27,596,925 | 21,264,151 | 19,223,470 | 7,904,996 | 759,658 |
M4 DS L | 26,706,483 | 22,914,129 | 20,900,171 | 8,748,394 | 179,474 |
M4 IRR R | 27,028,350 | 21,354,251 | 17,907,958 | 9,592,832 | 682,325 |
M4 DS R | 29,588,911 | 21,875,311 | 18,629,316 | 11,112,533 | 1,518,832 |
Sequences obtained after quality, adapter, and size (read lengths of less than 17 nucleotides) filtering. bSequences obtained after filtering noncoding RNAs (e.g. tRNA, rRNA, snoRNA, and snRNA). cTotal of unique sequences from conserved and novel miRNAs matching the grape genome (12X V1 GGDB CRIBI) and/or miRBase (version 21).