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. 2017 Feb 22;173(4):1940–1952. doi: 10.1104/pp.16.01489

Figure 5.

Figure 5.

Overexpression of 5′D8(+) by TYLCCNV-5′D8(+) in N. benthamiana. A, Scheme of 5′D8(+) and its related target mRNA sequences. B, The phenotype of overexpressing 5′D8(+) in N. benthamiana plants inoculated with TYLCCNV-5′D8(+). TYLCCNV empty vector-inoculated plants were used as negative control. Photographs were taken at 30 dpi. C, Relative expressions of 5′D8(+), pri-5′D8(+), and 5′D8(+) targeted ARF4 and ARF3 genes in plants inoculated with TYLCCNV or TYLCCNV-5′D8(+) by RT-qPCR. Data are presented as the mean of three independent experiments. The error bars indicate mean ± sd. UD, Undetectable. Asterisks indicate statistical significance (Student’s t test, P < 0.01). eIF4α was used as an internal control.