Silence of PCNA by TYLCCNV-amiRPCNA in N. benthamiana. A, Scheme of amiRPCNA and its related target mRNA sequence. B, PCNA-silencing phenotype in N. benthamiana plants inoculated with TYLCCNV-amiRPCNA. TYLCCNV empty vector-inoculated plants were used as negative control. Photographs were taken at 25 dpi. C, Relative expressions of amiRPCNA, pri-amiRPCNA, and PCNA in plants inoculated with TYLCCNV or TYLCCNV-amiRPCNA by RT-qPCR. Data are presented as the mean of three independent experiments. The error bars indicate mean ± sd. UD, Undetectable. Asterisks indicate statistical significance (Student’s t test, P < 0.01). eIF4α was used as an internal control.