K36M mutation induces pleiotropic developmental defects in Arabidopsis. A, Phenotypic analysis of transgenic plants expressing wild-type H3.3K36 3xFLAG (WT), heterozygous H3.3 K36M (K36M), homozygous H3.3 K36M (K36M hom), and sdg8 at 35 d after germination. B, K36M has a significantly lower number of leaves at bolting compared to the wild type (n > 30 plants). C, K36M shows significantly more rosette branches compared to the wild type (n = 30 plants). Rosette branches were measured from plants 7 d after main bolt production. D, K36M mutants show decreased silique length compared to the wild type. Student’s t test was used to calculate the P values (***P < 0.001).