Figure 2.

K36M alters the expression of development- and metabolism-associated genes. A, Volcano plot displaying significantly upregulated and downregulated genes in K36M compared to the wild type (red dots, P < 0.05). The x axis represents the log₂ value of K36M fragments per kilobase per million mapped reads/wild-type FPKM, and the y axis is the −log₁₀ of the P value for the significance of differential expression. B, Gene Ontology analysis of significantly upregulated and downregulated genes presented as the −log₁₀ of P value. C, Log₂ FPKM of genes important for flowering in wild type and K36M. Asterisk marks each gene that is significantly differentially expressed in K36M. D, Semiquantitative gel-based RT-PCR showing expression of flowering genes at 21 d after germination. E, Quantitative RT-PCR showing the expression of genes involved in floral development and carotenoid synthesis. Each bar is the average of three biological replicates. Significance was determined via Student’s t test (*P < 0.05, **P < 0.01). FPKM, fragments per kilobase per million mapped reads.