Figure 3.

K36M mutation exhibits a dominant-negative effect on endogenous H3K36 methylation. A, Immunoblotting of histone methylation in the wild type (wild-type H3.3 3xFLAG transgene), two independent transgenic lines of K36M (K36M-1 and K36M-2), and sdg8. B, Heat map showing histone modifications on H3.3 K27-R40 and H3.1 K27-R40 peptides by mass spectrometry analysis. Each row is calculated as the log₂ value of % abundance in K36M relative to % abundance in the wild type for a specific modification. The column on the right represents average % abundance of the same histone modifications detected in the wild type. Each modification is the average of triplicate technical replicates for K36M and the wild type.