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Tm-22 requires CC, NBS, and LRR domains for the PM localization. A, Schematic diagram of Tm-22 mutants used for cell fractionation. B, Cell fractionation analysis of Tm-22 or its mutants. The total proteins extracted from N. benthamiana leaves expressing myc-tagged Tm-22 mutants were fractionated by ultracentrifugation at 100,000g. The fractions were detected by immunoblotting with anti-myc and anti-H+-ATPase antibodies. Rubisco was stained by Ponceau S.
