Figure 2.
Molecular characterization of GmZF351. A, Schematic representation of GmZF351 structure. aa, Amino acids. B, GmZF351 expression in various organs of soybean plants. Error bars indicate sd (n = 3). C, Histochemical analysis of GmZF351 promoter activity. The GUS reporter is driven by the GmZF351 promoter in transgenic Arabidopsis plants. Three GUS-expressing lines were investigated, and all exhibit a similar pattern. Bars = 0.5 mm. D, Expression pattern of GmZF351 at six seed developmental stages. Error bars indicate sd (n = 3). E, Subcellular localization of the GmZF351 protein revealed by transient expression assay in tobacco leaves. Bars = 20 μm. F, Transcriptional activation activity of GmZF351 in the transient Arabidopsis protoplast assay. Error bars indicate sd (n = 4).