Figure 2.

FT genes are misregulated in photoperiod-insensitive early flowering pea mutants. Developmental regulation of FT genes and the floral marker PIM in NGB5839 (WT) and the ppd-3 mutant are shown in comparison to sn-4 and dne-1 mutants. Plants were grown under an 8-h photoperiod in growth cabinets and samples harvested weekly until the appearance of visible flower buds. Transcript levels were determined in dissected shoot apex or uppermost fully expanded leaflet tissue and are shown relative to the ACTIN reference gene. Each sample consisted of pooled material from two plants, and each data point represents the mean ± se for n = 2 to 3. Asterisks indicate differences between wild-type and mutant values where P < 0.05.