Table I. Relative amounts of 14C-labeled acyl groups incorporated into different lipid classes in C. sativa microsomal preparations.
Microsomal preparations were incubated with nonradioactive G3P and an equimolar amount of each 14C-labeled acyl-CoA. 0, Control; 1.2, amiDGAT1.2; P+1.2, PDAT+amiDGAT1.2. Numbers represent mean values ± sd of radioactivity in % (of cpm; n = 3). PC* also contains minor amounts of LPC. Assay conditions are described in “Materials and Methods.”
| Lipid |
TAG |
DAG |
PA |
PC* |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1.2 | P+1.2 | 0 | 1.2 | P+1.2 | 0 | 1.2 | P+1.2 | 0 | 1.2 | P+1.2 | |
| 18:1 | 26 ± 1 | 23 ± 4 | 25 ± 4 | 33 ± 4 | 41 ± 1 | 24 ± 2 | 26 ± 4 | 23 ± 2 | 21 ± 1 | 47 ± 5 | 44 ± 3 | 47 ± 3 |
| 18:2 | 25 ± 1 | 18 ± 4 | 27 ± 1 | 39 ± 6 | 34 ± 5 | 31 ± 3 | 47 ± 3 | 45 ± 1 | 43 ± 1 | 30 ± 2 | 29 ± 1 | 27 ± 2 |
| 18:3 | 49 ± 0 | 59 ± 5 | 49 ± 1 | 31 ± 2 | 25 ± 5 | 45 ± 2 | 27 ± 7 | 32 ± 2 | 36 ± 0 | 23 ± 5 | 27 ± 3 | 25 ± 2 |