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. 2017 Mar 1;173(4):2148–2162. doi: 10.1104/pp.16.01952

Figure 2.

Figure 2.

Recombinant prRBCS-GST binds to AtToc159G. A, AtToc159G was expressed and purified from E. coli. Protein integrity was analyzed by SDS-PAGE, and the gel was stained with Coomassie Blue. B, Purified AtToc159G was incubated with GST or prRBCS-GST. Proteins were pulled down by GSH resin and analyzed by SDS-PAGE. The bound proteins were visualized by immunoblotting using the anti-GST or anti-AtToc159G antibody as indicated. The anti-AtToc159G antibody was raised in rats against the purified AtToc159G protein as shown in A.