Figure 8.

Genotypic and phenotypic analyses of transgenic plants. A, Total genomic DNA was isolated from the ppi2 mutant, the corresponding wild-type plant (WT), and three transgenic lines containing the transgene encoding the different AtToc159GM variants transformed into the ppi2 mutant. DNA was amplified by PCR using primer pairs specific for the endogenous AtToc159 gene, the ppi2 T-DNA insertion, or the transgene encoding the introduced AtToc159GM. Primer positions are depicted at right with red arrows. The attB1 and attB2 sites resulting from the LR recombination reaction are shown in blue, and the FLAG tag from the pEarleyGate 202 vector is shown in green. B, Phenotypes of 12-d-old wild-type, AtToc159GM transgenic, and ppi2 mutant plants grown on Murashige and Skoog (MS) medium. C, AtToc159 protein expression. Protein extracts from 12-d-old plants as shown in B were analyzed by SDS-PAGE and immunoblotting using anti-AtToc159 antibody.