Figure 2.

Several membrane proteins were deficient in SCY2-RNAi plants. A, Total protein extracts from 6-d-old estrogen-induced seedlings were subjected to SDS-PAGE and immunoblotting with various antibodies. Actin was used as the internal control for equal protein loading. For better comparison, samples from empty vector-transformed plants were diluted to 0.5- and 0.25-fold. At the bottom, the blot for TIC40 was exposed to film for a longer time to show iTIC40 accumulation in SCY2-RNAi plants. i, iTIC40; m, mTIC40. B, Bar graph of the relative amounts of immunoblot signals of the proteins from A. Each bar presents the mean value of three biological replicates normalized to control plants (pMDC7). Error bars indicate se. C, Relative mRNA levels of TIC110, TIC40, and FTSH12 (left chart) and SCY1, TATC, and ALB3 (right chart) in 6-d-old seedlings germinated on MS medium containing 20 μm estrogen as determined by qRT-PCR analysis. Data represent mean values of at least four biological replicates normalized to control plants (pMDC7). Error bars indicate se.