Figure 6.

Comparison of Rubisco activity supported by NtRbcS-M and NtRbcS-T in C. reinhardtii and in tobacco. A, Specific activities of purified Rubisco from pSS1-CrRbcS1, pSS1-NtRbcS-M, or pSS1-NtRbcS-T C. reinhardtii transformants according to the pH values. Purified Rubisco (3 µg) was assayed for RuBP carboxylase activity in a buffer brought to the indicated pH. B, Relative activity of Rubisco from tobacco trichomes and leaves according to the pH. Soluble proteins from an extract from tobacco trichomes (30 µg) or leaf tissues clear of trichomes (15 µg) were assayed for RuBP carboxylase activity in a buffer brought to the indicated pH. The data represent the percentage of the maximal activity (at pH 8.0) for each sample. In A and B, the means ± the ses of four independent preparations are shown. Different letters indicate the significant differences between the transformants at a given pH according to a Kruskal-Wallis (A) or Mann-Whitney tests (B) followed by a Tukey post-hoc test (P < 0.05).