Figure 5.
Rescue of the morphological defects of hair bundles in pcdh15ath263b mutants by either isoform of Pcdh15a. Images of inner ear hair cells in live fish were obtained at 6 dpf. A, Wild-type hair bundles have a cone-shaped appearance. B, In the pcdh15ath263b mutants, the hair bundles are splayed and split away from the kinocilia (arrowheads). C, D, Morphological defects of mutant hair bundles were rescued with Pcdh15a-CD1-EGFP (C) or Pcdh15a-CD3-EGFP (D). Brackets indicate hair bundles with detectable GFP signal. n > 8 larvae for each genotype. Yellow scale bar, 5 μm.