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. 2017 Mar 22;37(12):3425–3439. doi: 10.1523/JNEUROSCI.2752-16.2017

Figure 6.

Figure 6.

Manipulations of endosomal and BMP trafficking alter Snx16 compartments and interact synergistically with HttQ138. A, Representative images showing changes in Snx16 distribution in ElavC155;UAS-Snx16-GFP/UAS-myrRFP control lines (A1) compared with manipulations of the indicated Rab proteins (A2: ElavC155;UAS-Rab7DN;UAS-Snx16-GFP, A3: ElavC155;UAS-Snx16-GFP/UAS-Rab11CA, A4: ElavC155;UAS-Snx16-GFP/UAS-Rab5CA, A5: ElavC155;UAS-Snx16-GFP/UAS-Snx16-GFP, A6: ElavC155;UAS-HttQ138-RFPmed/+; UAS-Snx16-GFP/+, A7: ElavC155;UAS-Rab7CA/+;UAS-Snx16-GFP/+, and A8: ElavC155;UAS-Rab11DN;UAS-Snx16-GFP). B, Quantification of Snx16-GFP fluorescence levels in the indicated genotypes. C, Representative images of synaptic growth in ElavC155;UAS-HttQ138-RFPmed/+;UAS-myrRFP/+ controls (C1) versus ElavC155;UAS-HttQ138-RFPmed;UAS-Rabs (C2: ElavC155;UAS-HttQ138-RFPmed/UAS-Rab7DN, C3: ElavC155;UAS-HttQ138-RFPmed/ UAS-Rab7CA, C4: ElavC155;UAS-HttQ138-RFPmed/+;UAS-Rab11CA/+, and C5: ElavC155;UAS-HttQ138-RFPmed/+;UAS-Rab5CA/+). D, Quantification of UAS-HttQ138-mRFPmed intensity from the genotypes shown in C. E, Quantification of satellite bouton number in the indicated genotypes from ElavC155-driven expression of the indicated transgenes. F, Quantification of the percentage of third-instar larvae surviving to adults in the indicated genotypes as above. ANOVA followed by Tukey's multiple-comparisons test was used for statistical analysis. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. Error bars indicate SEM.