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. 2017 Mar 29;37(13):3478–3490. doi: 10.1523/JNEUROSCI.3674-16.2017

Figure 4.

Figure 4.

Neurochemical phenotype of AT1aR-containing cells within the PVN. a–m, Coronal sections through the PVN of an AT1aR-tdTomato reporter mouse depicting tdTomato and either (a–d) CRH and TRH mRNA, (e–g) vGlut2 mRNA, (h–j) AVP, (k–m) OT, or (h–j) FG-labeled rostral ventrolateral medulla-projecting preautonomic neurons. a–d, Projection images of the PVN depicting (a) AT1a-Tom in magenta, (b) CRH mRNA in cyan, (c) TRH mRNA in green, and (d) the merged image. e–g, Projection images of the PVN depicting (e) AT1a-Tom in magenta, (f) vGlut2 mRNA in cyan, and (g) the merged image. h–j, Projection images of the PVN depicting (h) AT1a-Tom in magenta, (i) AVP in green, and (j) the merged image. k–m, Projection images of the PVN depicting (k) AT1a-Tom in magenta, (l) OT in green, and (m) the merged image. n–p, Projection images of the PVN collected from AT1aR-tdTomato mice that received FG injections into the RVLM and were perfused 7 d later depicting (n, q, t) AT1aR in magenta, (o, r, u) FG-labeled RVLM-projecting neurons in green, and (p, s, v) the merged images. 3v, Third cerebral ventricle. Scale bars: a–p, 100 μm; q–v, 10 μm.