Table 1.
Task sequence in each cohort
Lighting condition | Cohort | Recognition task sequence |
---|---|---|
Condition 1: LD | 1A (n = 8) | H × 6 → (1) object × 4 → (2) object displacement × 2 → (3) object-in-place × 2 |
1B (n = 8) | H × 6 → (1) object × 4 → (2) object displacement × 2 → (3) object-in-place × 2 → (4) odor × 4 | |
1Ca (n = 8) | H × 6 → (1) object × 4 → (2) object displacement × 2 → (3) object-in-place × 2 | |
Condition 2: rLL | 2Ab (n = 8) | H × 6 → (1) object displacement × 2 → (2) object-in-place × 2 → (3) odor × 4 |
2Bc (n = 8) | H × 6 → (1) odor × 4 → (2) object displacement × 2 → (3) object-in-place × 2 → (4) object × 4 | |
2Cb (n = 8) | H × 6 → (1) object displacement × 2 → (2) odor × 2 | |
Control LL | 3A (n = 16) | H × 6 → (1) object × 2 |
3B (n = 8) | H × 6 → (1) object-in-place × 2 | |
3C (n = 16) | H × 6 → (1) object × 2 (LD) → (2) odor × 2 (LL) |
All mice received 6 habituation trials (H) in the empty arena (3 trials at ZT 6 and 3 trials at ZT 18 under LD) before recognition testing. In each cohort, half of the recognition trials were given at ZT/CT 6 and the remaining trials were given at ZT/CT 18.
aSample–test delay was 24 h for cohort 1C; for all other cohorts, the sample–test delay was 5 min.
bCohorts 2A and 2C experienced one cycle of LD–LL alternation before recognition testing.
cCohort 2B experienced four cycles of LD–LL alternation before recognition testing.