Fig. S8.

The effects of PERKi and NAC treatment on glucose starvation-induced cell death. (A) The effect of PERK inhibitor on glucose deprivation-induced apoptosis in 786-O stable cells. 786-O stable cells were cultured in glucose-free medium with 5-nM PERK inhibitor (−Glc +PERKi) or vehicle (−Glc) for 24 h, then assayed for cell apoptosis by FACS. (B) The effect of antioxidant NAC on glucose deprivation-induced apoptosis in UMRC6 stable cells. Cells were cultured in glucose-free medium with 5 nM NAC (−Glc +NAC) or vehicle (−Glc) for 14 h, then assayed for cell apoptosis by FACS. *P < 0.05; **P < 0.01; ns: nonsignificant.