Fig. 5.
Niche independence is a key contributor to metastatic growth. (A) Mutant human colon organoids were dissociated, and subsequently 50,000 cells were intrahepatically injected in mice. The niche was reconstituted by overexpression of Noggin. Tumor growth was monitored by bioluminescence. (B) The kinetics of metastatic growth. Points are averages, and error bars indicate SEM of biological replicates. Quadruple n = 3, TripleSMAD4WT and TripleSMAD4WT overexpressing Noggin n = 5. (C) Representative images at indicated timepoints.