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. 2017 Mar 7;114(12):3204–3209. doi: 10.1073/pnas.1700695114

Fig. 4.

Fig. 4.

Examples of cell resuscitation of individual P. aeruginosa (pMF230) cells within 15-μm-diameter oil drops with visualization by CSLM. Cells were incubated in PBS for 4 d, and encapsulated in drops containing TSB medium. (A) P. aeruginosa PAO1 following 4 d of starvation and then regrowth for 24 h in TSB. (B) P. aeruginosa PAO1 ∆hpf after 4 d of starvation and then regrowth for 24 h in TSB. (Insets) Cell regrowth and single nondividing cells in drops. (C) Percentage of cells that replicated inside of TSB-containing drops versus cells that remained as single intact nondividing cells following starvation in PBS. Cell counts of replicating and nonreplicating cells are shown in SI Appendix, Fig. S9. Regrowth of PAO1 and the ∆hpf mutant cells in drops over 24 h is shown in Movies S1 and S2.