Fig. S5.

ChIP analysis indicating that ZPO2 and ZBTB32 occupy the Gata3 promoter. EpH4.9 cells were grown in 150-mm culture dishes and cotransfected with full-length Zpo2 and Myc-tagged ZBTB32 (generous gifts from Dr. I.-Cheng Ho, Harvard Medical School) constructs via FuGENE6 transfection reagent (Promega). ChIP analysis was performed using the ChIP-IT High-Sensitivity Kit (Active Motif) in accordance with the manufacturer’s protocol. qPCR for ChIP samples was performed using Gata3 promoter sequence primers listed in the text. ChIP analysis indicated that both ZPO2 and ZBTB32 occupy the promoter sequence of GATA3.