Fig. S4.

(A) Experimental scheme. (B) The numerical kinetics of splenic OT-II T cells in the adoptive transfer model. RBC–OT-II leads to OT-I T-cell disappearance after an early spurt of proliferation as analyzed by flow cytometry. (C) CFSE-dilution indicates the proliferation of OT-II T cells at day 3 upon treatment administration. Frequency of splenic (D) CD44⁺ and CD62L⁺ OT-II T cells, (E) IFN-γ⁺ and TNF-α⁺ OT-II T cells, and (F) PD-1⁺, TIM-3⁺, LAG-3⁺, and Fas⁺ OT-II T cells in the spleen at day 3 upon transfusion. (G) Frequency of splenic Foxp3⁺ CD4⁺ regulatory T cells at day 10 after CFA/OVA challenge. *P < 0.05; **P < 0.01.