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. 2017 Mar 6;114(12):E2533–E2539. doi: 10.1073/pnas.1616493114

Fig. 4.

Fig. 4.

Auxin response cell-autonomously controls ground and vascular tissue initiation. (A) Ratio of DII-3xYFP and mDII-tdTomato proteins in globular-stage R2D2 embryo in longitudinal (A) or transverse (Inset in A) cross-section. Ratio (tdTomato/YFP) is shown on a false color scale with red indicating high and blue, low ratio. (B) Quantification of the mDII/DII fluorescence intensity ratio in nuclei of protoderm (P), ground tissue (G), and vascular tissue (V) cells in globular-stage embryos (n = 14 embryos; error bars are SEM). (C and D) Expression of DR5v2-ntdTomato in globular-stage embryo in longitudinal (C) and transverse (D) cross-sections. False color scale is red for high fluorescence signal and blue for low signal. White counterstain is Renaissance RS2200 signal. (E) Expression of GFP in a globular-stage embryo of the pMGP>>GFP driver line. (F) Divisions in a globular-stage pMGP>>bdl F1 embryo. (G) Expression of GFP in a globular-stage embryo of the pTMO5>>GFP driver line. (H) Divisions in a globular-stage pTMO5>>bdl F1 embryo. Red counterstaining in E and G is Renaissance RS2200 signal. Ground tissue divisions are indicated by green arrowheads, vascular divisions by red arrowheads, and hypophysis division by white arrowheads in F and H. (I and J) Radial and (K and L) longitudinal cross-sections of basic fuchsin-stained pTMO5>>GFP (I and K) and pTMO5>>bdl (J and L) roots. Intense central signal in I and J represents protoxylem, and patches surrounding the vasculature represent Casparian strips. Lignified Casparian strips are indicated by asterisks in K and L.