Table 2. Mouse embryonic stem cells: top 10 Xist-binding proteins from RAP-MS 23.
| Crosslinked
proteins |
In order of
abundance |
Protein structural
motifs |
Length
(number of amino acids) |
|---|---|---|---|
| SHARP
(SPEN) |
1 | 3 RRM, SPOC | 3,640 |
| RBM15 | 2 | 3 RRM, SPOC | 969 |
| MYEF2
(hnRNP M) |
3 | 3 RRMs, homology to
hnRNP M4 |
591 |
| CELF1 | 4 | 3 RRMs | 486 |
| hnRNP C | 5 | 1 RRM | 313 |
| LBR | 6 | Chromatin-interaction
domain, transmembrane region, lamin-interacting domain |
626 |
| SAF-A
(hnRNP U) |
7 | RGG, SPRY domain,
ATPase domain |
793 |
| RALY
(hnRNP C) |
8 | 1 RRM | 312 |
| hnRNP M | 9 | 3 RRM | 729 |
| PTBP1
(hnRNP I) |
10 | 4 RRM | 555 |
hnRNP, heterogeneous nuclear ribonucleoprotein particle; RAP-MS, RNA antisense purification-mass spectrometry; RRM, RNA recognition motif; SILAC, stable isotope labeling by amino acids in culture; SPOC, Spen paralog and ortholog C-terminal domain.