Figure 4.

Levels of NADPH, activity of GR and GPx, and GSSG/tGSH in the mitochondria from Idh2^+/+ and Idh2^−/− mice after I/R. Idh2^+/+ (Idh2 WT) and Idh2^−/− (Idh2 KO) mice were subjected to either 25 minutes of bilateral renal ischemia or sham surgery. (A) Kidney sections were subjected to immunohistochemical staining using an anti-NAPDH antibody. Images were obtained from the outer medulla. Upper panels are at low magnification, lower panels are at high magnification of the dash-lined rectangle in upper panel. Brown indicates NADPH-positive signal. (B) Activity of GR, (C) GSSG/tGSH, and (D) the activity of GPx in the mitochondrial fractions from kidneys were determined as described in the Concise Methods section. Results are expressed as the mean±SEM (n=6). *P<0.05 versus respective sham-operated mice; ^†P<0.05 versus ischemia-operated Idh2 WT. KO, knockout; WT, wild type.