Figure 5.

Expressions and activities of cytosolic GR, GPx, G6PD, catalase, and GSSG/tGSH in the cytosol of Idh2^+/+ and Idh2^−/− mice after I/R. Idh2^+/+ (Idh2 WT) and Idh2^−/− (Idh2 KO) mice were subjected to either 25 minutes of bilateral renal ischemia or sham surgery. Activities of GR (A), GPx (C), G6PD (D), catalase (E), and GSSG/tGSH (B) in the cytosol fraction were determined. (F) Kidney samples were subjected to Western blot analysis using anti-G6PD and anti-catalase antibodies. GAPDH was used as loading control. (G and H) Densities of blots were measured using ImageJ software. Results are expressed as the mean±SEM (n=6). *P<0.05 versus respective sham-operated mice. Cat, catalase; Cyto, cytosolic; Isch, ischemia; KO, knockout; WT, wild type.