Figure 3. The circadian clock is unaffected by lung adenocarcinoma.

A) Heatmap for ‘BOTH’ category genes that are unaltered in expression between WT and TB mice. B) GO pathway analysis using biological process for ‘BOTH’ oscillating genes. C) BMAL1 protein phosphorylation by western and circadian expression of the clock genes, Bmal1, Clock, Rev-erbα, Dbp, Per2 and Cry1, as determined by quantitative real-time PCR (RT-PCR). D) Locomotor activity analysis for WT and TB mice, as calculated by the free-running period (Tau) in dark/dark (D/D) conditions. An n=12 WT and n=12 TB mice were used for behavioral analysis. E) Food intake of WT and TB mice shown over a 48-hour period (left panel). Total food intake was normalized to body weight of each animal. An n=7 WT and n=8 TB mice were used for indirect calorimetry analysis. F) VCO₂/VO₂ is shown as the respiratory exchange ratio (RER) for WT and TB mice over a 48-hour period. Average RER is quantified during the light and dark phases (right panel). Error bars indicate standard error of mean (SEM). Significance was calculated using Student’s T test and * and ** indicate p-value cutoffs of 0.001 and 0.0001, respectively.