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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Plast Reconstr Surg. 2017 Apr;139(4):900e–910e. doi: 10.1097/PRS.0000000000003187

Figure 1.

Figure 1

Comparison of TPL in GNS vs. Qtracker labeled ASCs. Undifferentiated ASCs are shown stained with Hoechst33342 following a 24 hour incubation with 0.14nM GNSs, and imaged with MPM (top, scale bars = 20 μm). The cytoplasmic localization of the GNSs (yellow arrows), in relation to the nucleus that is stained blue is shown following imaging with MPM. Undifferentiated ASCs are shown following a 24 hour incubation with 0.14nM GNSs and 0.14nM Qtracker (bottom, scale bars = 50μm). Cells were fixed on days 1, 2, and 4 and then imaged to determine the relative fluorescence of each optical label. Each cell could be roughly distinguished by its predominant fluorescence. Qtracker (red) and GNSs (white) could be seen in each cell. Fluorescence was measured over a period of 4 days. Calculated total cell fluorescence (CTCF) was calculated with ImageJ and analyzed using a two-way ANOVA. There was a significantly greater degree of fluorescence emitted by those cells labeled with GNSs vs. Qtracker throughout all 4 days of cellular proliferation. (P<0.0001). MPM images were taken at 800nM using a laser power of 3.7mW.