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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Plast Reconstr Surg. 2017 Apr;139(4):900e–910e. doi: 10.1097/PRS.0000000000003187

Figure 2.

Figure 2

ASC adipogenic differentiation following labeling with GNSs and Qtracker. For all studies, ASCs were incubated with 0.14nM GNSs and 0.14nM Qtracker for 24 hours prior to differentiation. In columns 1 and 2, for adipogenesis on the 7th and 21st day of differentiation, images were taken with MPM to monitor the TPL of the labels (scale bars = 50μm). Images in the third column show labeled ASCs on the 21st day of differentiation at 5× zoom with GNSs and Qtracker (scale bars = 25μm). Images in the third column were stained with Hoechst33342. The last column represents the confirmation of phenotypic differentiation with Oil Red O for adipogenesis (scale bars = 100μm). For adipogenesis, the cells labeled with GNSs could be easily visualized over a period of 21 days and to a greater extent than with the Qtracker label. Results similar to those shown in this figure were seen in osteogenesis and chondrogenesis as well. All samples were imaged with MPM using a wavelength of 800nM at 3.7mW.