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. 2017 Mar 30;12(3):e0174374. doi: 10.1371/journal.pone.0174374

Fig 8. UBC9 knockdown influences the expression of multiple downstream genes.

Fig 8

(A) Representative images of 3 independent experiments with similar results are presented. Downregulation of UBC9 expression decreases the expression of the P-p65 and P-IκBα proteins, but increased the expression of the IκBα protein. (B) Graph of the relative ratios of the P-p65, p65, P-IκBα and IκBα proteins to β-actin in each group. *P < 0.01 and #P <0.05 compared with NC shRNA group, n = 4.(C) The subcellular localization of p65 in activated LX-2 cells treated with UBC9 shRNA were examined by confocal microscopy analysis with a confocal microscope.