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Effect of the gradient time (a) and of the linear flow velocity (b) on the peak capacity of the 1D HILIC separations. Conditions: mobile phase A: 94% ACN, 5% THF 1% 12.5 mM ammonium acetate; mobile phase B: 12.5 mM ammonium acetate, Vinj = 1 μL, T = 15 °C, UV detection at 220 nm. (a) Linear gradient from 0 to 30% B at u = 2.4 mm/s. (b) Linear gradient from 0 to 30% B with constant tG/t0 of 25.
