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. 2015 Dec 28;88(3):1785–1793. doi: 10.1021/acs.analchem.5b04051

Figure 4.

Figure 4

Dependence of the peak capacity on the gradient time (a) and on the dilution factor (b) of the 2D RP separation for the three C18 columns considered in this study: (blue square) 50 × 4.6 mm; 2.6 μm particles (red circle) 50 × 2.1 mm; 1.7 μm (black triangle) 50 × 1 mm; 1.9 μm. Conditions: mobile phase A: 12.5 mM ammonium acetate + 1% 1-BuOH; mobile phase B: MeOH + 1% BuOH. Gradient from 35% to 100% with times scaled for different tG/t0 (see section S5 of Supporting Information); u = 7.2 mm/s, T = 50 °C, Vinj = 1 μL of Agnique TSP 16A in 50/50 ACN/H20.