Skip to main content
. 2017 Mar 30;12(3):e0174689. doi: 10.1371/journal.pone.0174689

Fig 1. Encapsulation of Cell-Free Expression (CFE) and formation of lipid vesicles.

Fig 1

(A) HeLa mammalian CFE system is a combination of HeLa lysate, Mix 1 buffer, GADD34, T7 RNA polymerase, Mix 2 accessory factors and DNA plasmid containing T7 promoter. (B) Thin double emulsions are formed in the glass capillary microfluidic device. (C) Image of thin double emulsion formation using the glass capillary microfluidic device. (D) Lipid vesicles are formed by first forming aqueous-in-oil-in-aqueous double emulsions where phospholipids are dissolved in volatile middle phase organic solvents. CFE system was encapsulated with surfactant (PVA/pluronic) and fluorinated surfactant (F6-TAC/F8-TAC) inside the lipid vesicles. Phospholipids self-assemble to form phospholipid monolayers on the aqueous-oil and oil-aqueous interfaces. Upon solvent evaporation, lipid vesicles form.