Fig 2. Aggregate was observed when CFE is encapsulated in the vesicle.

(Top) Buffer solution (20 mM HEPES pH 7.5, 400 mM sucrose, 2% PVA, 8% PEG) was encapsulated as the inner phase using capillary droplet microfluidics with DOPC/cholesterol dissolved in chloroform/hexane as the middle phase (see materials and methods) (Bottom) HeLa CFE (18 μl HeLa lysate, 4.5 μl Mix 1 buffer, 5.4 μl GADD34, 4.5 μl T7 RNA polymerase, 3.6 μl Mix 2 accessory factors and 3 μl MscL DNA plasmid containing T7 promoter) with 2% PVA was encapsulated with DOPC/cholesterol dissolved in chloroform/hexane as the middle phase and incubated at 32 degrees in a closed environment.