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. 2017 Mar 30;12(3):e0174689. doi: 10.1371/journal.pone.0174689

Fig 6. High concentration of mammalian Hela lysate is prone to aggregation when exposed to surfactant.

Fig 6

(A) (Top) Representative brightfield images of different dilution of CFE reaction with 2% PVA under bulk condition. (Bottom) Representative brightfield images of single emulsions encapsulating 2% PVA at different CFE dilutions. Yellow arrowheads denote the appearance of aggregates. (B) (Left) eGFP expression in HeLa CFE as a function of CFE dilution measured in microwell plates (n = 4, ±S.E.), unpaired t test comparing with 1X; ***: p < 0.0001. (Right) eGFP expression in HeLa CFE at different volume ratios between lysate and Mix 1 measured in microwell plates (n = 4, ±S.E.), unpaired t test comparing with 9:2.25 volume ratio; ***: p < 0.0001.